Choose enzymes manually by name, or by editing an existing enzyme set or using search criteria.
Choose an Enzyme Set
To choose an existing enzyme set, click Enzymes → Choose Enzymes.
In the "Choose Enzymes" dialog, select the enzyme set name in the Choose from: list.
If required, click Remove All to clear the existing "chosen Enzymes", or select and remove individual "Chosen Enzymes".
Choose and Add an Enzyme by Name
To search within the chosen enzyme set, enter the enzyme name in the search box.
Alternatively, click on an enzyme name to select it.
Add to an Enzyme Set
To add the selected enzyme(s) to the Chosen Enzymes set, click the Add → button.
To add all of the enzymes from the original set to the Chosen Enzymes set, click the Add All → button.
Remove from an Enzyme Set
To remove the selected enzyme from the Chosen Enzymes set, select the site then click the Remove button or press the Delete key.
To remove all of the enzymes from the Chosen Enzymes set, click the Remove All button.
Choose Enzymes by Criteria
To choose enzymes based on criteria, click the "Choose from" check box. Enzymes that meet the selected criteria will be highlighted in the Choose from: list.
- Use the Choose from dropdown to specify the enzyme set from which the enzymes will be chosen.
- Use the Enzymes cut dropdown to specify where the chosen enzymes will cut (anywhere, inside selection, outside selection).
- Use the fields to specify the number of allowed cut sites in the sequence.
- Specify the type of end generated (blunt, stick, degenerate, 5' overhang, 3' overhang, nick).
- Specify the allowed recognition sequence length, and structure (palindromic, uninterrupted, nondegenerate).
- Specify how the enzyme cuts the sequence.
