How do I add an intramolecular bond Feature to a protein sequence?
SnapGene allows the addition of intramolecular bond Features to a protein sequence. Bond types can be disulfide, thioether, thiolester, x-link or other. In this lesson we show how to add a disulfide bond.
Open a Protein Sequence
Open a protein sequence. In this example a cysteine-cysteine disulphide bond is created.
Click and drag to select the region with the two residues that will share a covalent bond as the first and last residue in the selection.
Alternatively, click to position the cursor to the left of the first residue, then shift-click immediately to the right of the second residue to select the region.
The first and last residues in the selection will be joined by the bond.
Add a Bond Feature
Click Features → Add Feature.
Enter a name for the Feature.
Set the Feature Type to "Bond", and set the Subtype.
Optional: add information to the /note qualifier.
Click OK to add the bond feature.
View the Bond Feature
The bond Feature displays in Map view and Sequence view as a line joining the specified amino acids.
Double click on the bond line if you want to edit the bond Feature