How can I import primers from one file into another?
Open the Destination File
Open a target sequence file that will receive the imported primers.
SnapGene will import only primers that are calculated to be able to hybridize to the target sequence. To change hybridization settings for any sequence, see this article – Adjust Primer Hybridization Parameters.
If the destination file already has primers, SnapGene will not duplicate existing primers, but will offer the option of importing duplicate primers.
Primers can be imported from:
- a source Snapgene .dna file
- a delimited list in .txt .csv, .tsv or .rtf format
- a Vector NTI® database or oligo archive
- a Clone Manager primer archive
Import from a SnapGene .dna File
To specify the source file, click Primers → Import Primers → Import Primers from a SnapGene File.
In the "Import Primers from a SnapGene file" dialog, use the dropdown, or use the Browse button to choose a *.dna source file, then click OK.
Import from a Source List
To specify the source list, click Primers → Import Primers → Import Primers from a List... .
In the "Import Primers from a List" dialog, choose a source file in .txt, .csv, .tsv or .rtf format, or choose Import primers copied to the clipboard, then click OK.
Import from a Vector NTI® File
To specify the source Vector NTI® file, click Primers → Import Primers → Import Primers from Vector NTI®....
In the "Import Primers from Vector NTI®" dialog, choose a database or oligo archive, then click OK.
Import from a Clone Manager File
To specify the source Clone Manager file, click Primers → Import Primers → Import Primers from Clone Manager... .
In the Import Primers from Clone Manager dialog, choose a *.pd4 or *.px5 file, then click OK.
View the Matching Available Primers List
A second dialog will list the primers that will bind to the target and can be imported. If desired, click the check box at the lower left to hide or show full descriptions.
The left-hand Add column indicates which primers can be imported. To change the default choices, click the relevant check boxes, then click Add [N] Primer(s).
View the Imported Primers
The imported primers will be added to the destination file.
