How can I use SnapGene to calculate the molecular weight, mass, nucleotide content, concentration or copy number of a DNA or RNA sequence?
Open a DNA Sequence
Open a DNA sequence of interest.
If you wish to calculate information for a portion of the sequence then select the region. In the above screenshot the mEGFP feature has been selected.
Show DNA Calculations
SnapGene calculates MW based on the MW of individual nucleotides in the sequence and adjusts MW for end modifications (5'-phosphorylation, unphosphorylated, or covalently closed ends).
Sequence ambiguities (for example, Y = C or T) will be calculated as the average MW for the possible nucleotides at that position.
Click Tools → Show DNA Calculations.
1. Calculations for the full length sequence are shown at the top of the window.
2. Calculations for a selected region are shown (if a region is selected) at the bottom of the window.