How do I import a multi-sequence format file (fasta or GenBank) into SnapGene 7.0 or later?
GenBank, fasta and fastq† files can contain multiple sequences in a single file. You can import these file types into SnapGene as a new Project folder, or Import them into an existing Project folder.
†SnapGene can import small fastq format files (10-100 reads) as individual files. Snapgene is not designed to import or use NGS fastq datasets.
Import a Multisequence File as a New Project
Start with a multi-sequence .fasta or .genbank file.
In SnapGene click menu File → Open, or click "Open File..." in the main window.
Locate the Fasta (.fa or .fasta) or GenBank (.gb) file in the file picker.
Select the file and click Open.
SnapGene will parse the file and identify all sequences present in the file.
- Set a name for the new Project folder.
- Set the location to create the new Project folder.
- Check the checkboxes to "Circularize" if one or more sequences are expected to be circular (note, SnapGene will identify likely plasmids and automatically check to option to circularize).
- Check the option to "Automatically annotate common features" to have SnapGene detect and annotate any known common features (see Detect Common Features).
SnapGene will import the sequences and open the new folder as a project.
Import a MultiSequence File into an Existing Project
Open a folder as a project.
Click the Ellipsis and choose "Import Files...".
Locate the file in the file picker and click Open.
The sequences will be converted to separate .dna files and saved in the Project.
If you have imported a .fasta file then use Detect Common Features to annotate the imported files.
