How do I search Bioregister (BLAST search) for a similar sequence and import "hit" sequences into SnapGene?
Search Bioregister for a Similar Sequence
Log in to your Bioregister account before registering an entity as described in Authenticate with Dotmatics Bioregister.
Note, to use search your Bioregister version must be 6.1.0 or later.
Click on File → Import → Dotmatics Bioregister Sequences to start a search for a sequence.
Alternatively, on the SnapGene top toolbar click Import → Dotmatics Bioregister Sequences.
Manually Enter a Query Sequence
Select Search Dotmatics Bioregister for to Sequence and type or copy and paste a sequence into the Sequence field.
Use a SnapGene Sequence as a Query Sequence
Select an area of interest in an open SnapGene file and click Tools → BLAST Selected Sequence in Bioregister → DNA Database (alternatively, RNA or Protein database):
The system pre-populates a Sequence field from the selection:
Search in Bioregister
Choose whether to search the DNA, Protein or RNA database.
If necessary change sorting criteria based from E-value (default) to Score, Identity, Positives or Gap.
Click on Search Bioregister button.
Explore the Search Results
For every sequence "hit" detected in Bioregister, view the following information:
- Bioregister ID and Name
- Similarity parameters (Score, E-value, Identities, Positives, Gaps)
View or hide individual hit alignments by toggling the disclosure buttons.
Open or save a sequence
Select one or more sequences of interest by selecting a checkbox.
Select Open and click on the Import button to open the selected sequences in SnapGene. The Accession Number field in the Description Panel will contain the Bioregister ID. Please note, that the standard Accession Number link points to the NCBI database and not Dotmatics Bioregister.
Alternatively, select Save and click on the Import button to save a sequence to disk. The system will ask to select an appropriate location to save the files.