How do I insert a back-translated protein into a DNA sequence?
Open a DNA Sequence File and Define the Insertion Point
Open a DNA sequence and switch to Sequence view.
Click at the position you wish to insert the sequence, or select 1 or more nucleotides if you wish to replace a region with the feature. In the above example, the eGFP CDS stop codon will be replaced with the incoming sequence.
Specify the Protein Sequence and Back Translation Parameters
Click menu Edit → Insert → Reverse Translation....
- Type or paste the desired protein sequence into the field provided.
- Use the drop-down menu to specify the genetic code to use for back-translation.
- Choose whether alternate synonymous codons are chosen a) Randomly, b) with degeneracies, or c) using a specific codon usage table.
- Choose whether to add the new sequence as a translated feature.
Click Replace (or Insert) to add the new back translated sequence to your sequence.
If the option to "Add a translated feature" was checked, then give the feature a name and click OK.
View the Newly Added Sequence
Select the "Show Colors" button in the side toolbar to highlight (in red) the newly added sequence.
Save the modified sequence.
If you wish to add multiple sequence elements to an existing sequence then consider using Tools → Design Synthetic Construct...