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Primers

  • How does SnapGene estimate oligonucleotide (primer) melting temperatures (Tm)?
  • Can I configure SnapGene to show primer Tm values suitable for PCR polymerases such as Phusion®, Phire®, and Q5®?
  • I have a list of primers in an Excel file. Can I import those primers into SnapGene?
  • My primer shows up at irrelevant binding sites, or does not show up at the binding sites of interest. How can I adjust the number of binding sites?
  • What is the Red Squiggle I See When I Create or Edit a Primer?
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