FAQ

General

• Download the SnapGene User Guide as a PDF
• Can I use Greek Characters or Symbols in SnapGene?
• Where do I find the SnapGene End User License Agreement (EULA)?
• What are the System & Hardware Requirements for SnapGene?
• Will SnapGene run on Windows for ARM?
• Can I try a demo version of SnapGene?

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Licenses

• Error Code 99: Unable to contact registration server. Please try again later.
• My computer died. How do I transfer the license to a new computer?
• How do I transfer a license from one computer to another?
• If multiple accounts on the same computer are using SnapGene, does each account need a separate license?
• With a multi-license pack, can I use some licenses with macOS and others with Windows?

Files

• How do I back up my SnapGene files?
• Can I use store/use/share a SnapGene Collection on a network or Cloud drive?
• What is the maximum size for a SnapGene File?
• Can SnapGene read files created by my current molecular biology software?
• How can I copy a sequence from a file and preserve the history?
• Will I lose data if my subscription expires?

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Importing Files

• How do I import DNA, RNA, Protein or Alignment Files from other software?
• Why can't I import sequences from NCBI (GenBank)?
• Can I import an NCBI Assembly?

Primers

• How does SnapGene estimate oligonucleotide (primer) melting temperatures (Tm)?
• Can I configure SnapGene to show primer Tm values suitable for PCR polymerases such as Phusion®, Phire®, and Q5®?
• I have a list of primers in an Excel file. Can I import those primers into SnapGene?
• My primer shows up at irrelevant binding sites, or does not show up at the binding sites of interest. How can I adjust the number of binding sites?

Restriction Enzymes

• Why is the "Show Enzymes" Button Missing for my DNA Sequence?
• How can I selectively display restriction enzymes from my favorite supplier?
• How can I see a list of enzymes that don't cut my sequence?

Features

• How can I divide a feature into segments?
• How do I display introns?

Translations

• How can I obtain a protein sequence from a SnapGene file?

Gels

• How does SnapGene estimate supercoiled DNA migration rates in an agarose gel simulation?

Alignment

• How does SnapGene calculate conservation scores for a protein multiple alignment?

Sequence Traces

• What base caller does SnapGene use for sequence traces?

History

• Why has a files History information disappeared?

Printing and Exporting

• Can I export my Map, Sequence or Alignment as a vector-based graphic?
• Why do I see missing or garbled text after printing to a PDF file?

Citing SnapGene

• How should I cite SnapGene or SnapGene Viewer?

Troubleshooting

• Error Message: Why do I See the Message "Error opening file for writing:" When installing SnapGene for Windows?
• SnapGene 5.2.5 for Windows can't detect Common Features
• Automatic Update Issue: SnapGene 5.2.1 for macOS
• Error: "Sorry, unable to obtain a shared key from the keyserver because KeyAccess is not installed"
• Why won't SnapGene start on Ubuntu?